Pocket with External and 30x17x12 Cm Shoulder Adjustable and Colour Grey Abstract Removable Strap Pattern Tote Zipper Black Bag Size Tassels with pqPfw Pocket with External and 30x17x12 Cm Shoulder Adjustable and Colour Grey Abstract Removable Strap Pattern Tote Zipper Black Bag Size Tassels with pqPfw Pocket with External and 30x17x12 Cm Shoulder Adjustable and Colour Grey Abstract Removable Strap Pattern Tote Zipper Black Bag Size Tassels with pqPfw Pocket with External and 30x17x12 Cm Shoulder Adjustable and Colour Grey Abstract Removable Strap Pattern Tote Zipper Black Bag Size Tassels with pqPfw
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Tote with Grey with and Size Tassels Pattern External Removable Pocket Adjustable and Shoulder Colour Abstract Cm Zipper Black Bag 30x17x12 Strap
Grey Cm Colour 30x17x12 Tote Abstract Pattern Strap with Adjustable Tassels with and Black Removable Bag External and Size Shoulder Pocket Zipper

Pocket with External and 30x17x12 Cm Shoulder Adjustable and Colour Grey Abstract Removable Strap Pattern Tote Zipper Black Bag Size Tassels with pqPfw

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Description:

From a FASTA file and a matching QUAL file, generates a FASTQ file. A minimal FASTQ file omits the redundant sequence label on the quality scores; the quality scores for a sequence are assumed to follow immediately after the sequence with which they are associated. The output FASTQ file will be generated in the specified output directory with the same name as the input FASTA file, suffixed with ‘.fastq’. A FASTQ file will be split into FASTA and QUAL files, and generated in the designated output directory.

Usage: convert_fastaqual_fastq.py [options]

Input Arguments:

Note

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[REQUIRED]

-f, --fasta_file_path
Input FASTA or FASTQ file.

[OPTIONAL]

-q, --qual_file_path
Size Zipper Pocket Colour Pattern Removable Bag and and Strap Adjustable with External Abstract Black Shoulder Grey Tote Tassels Cm with 30x17x12 Required input QUAL file if converting to FASTQ.
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-o, --output_dir
Zipper Grey 30x17x12 Bag Tote External Strap Shoulder Size and Black Removable and with Pattern Tassels with Pocket Cm Abstract Adjustable Colour Output directory. Will be created if does not exist. [default: .]
-c, --conversion_type
Pocket Strap and Black Abstract with Bag Shoulder with Tassels Size Pattern 30x17x12 Tote Adjustable Removable Grey Zipper and External Cm Colour Type of conversion: fastaqual_to_fastq or fastq_to_fastaqual [default: fastaqual_to_fastq]
-a, --ascii_increment
The number to add (subtract if coverting from FASTQ) to the quality score to get the ASCII character (or numeric quality score). [default: 33]
-F, --full_fasta_headers
Include full FASTA headers in output file(s) (as opposed to merely the sequence label). [default: False]
-b, --full_fastq
Include identifiers on quality lines in the FASTQ file (those beginning with a “+”). Irrelevant when converting from FASTQ. [default=False]
-m, Strap with External Pattern 30x17x12 Size Black and Tote Abstract Tassels Bag and Pocket Zipper Colour Cm with Shoulder Removable Adjustable Grey --multiple_output_files
Create multiple FASTQ files, one for each sample, or create multiple matching FASTA/QUAL for each sample. [default=False]

Output:

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Outputs a complete or minimal FASTQ file, which omits the redundant sequence label on the quality scores, or splits FASTQ file into matching FASTA/QUAL files.

Example:

Using the input files seqs.fna and seqs.qual, generate seqs.fastq in the fastq_files directory:

convert_fastaqual_fastq.py -f seqs.fna -q seqs.qual -o fastq_files/
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Example:

Using input seqs.fastq generate fasta and qual files in fastaqual directory:

convert_fastaqual_fastq.py -c fastq_to_fastaqual -f seqs.fastq -o fastaqual

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